Isolation of SIBLING Proteins from Bone and Dentin Matrices. Academic Article

abstract

• The extracellular matrix of the bone and dentin contains several non-collagenous proteins (NCPs). One category of NCPs is termed the SIBLING (small integrin-binding ligand, N-linked glycoprotein) family, which includes osteopontin (OPN), bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein (DSPP), etc. These proteins have abundant phosphoserines, aspartic acids, and glutamic acids. In this protocol, we describe the extraction of NCPs from the bone and dentin matrices using guanidine-HCl/EDTA and the isolation of polyanionic SIBLINGs from NCPs using ion-exchange fast protein liquid chromatography (FPLC) to separate the differentially charged proteins into different fractions through a gradient elution by NaCl.

authors

• Wang, Xiaofang

published proceedings

• Methods Mol Biol

author list (cited authors)

• Wu, J., & Wang, X.

citation count

• 0

complete list of authors

• Wu, Jingyi||Wang, Xiaofang

publication date

• January 2019

publisher

• Springer Nature  Publisher

keywords

• Animals
• Bone And Bones
• Chromatography, Ion Exchange
• Dentin
• Edetic Acid
• Equipment Design
• Extracellular Matrix
• Extracellular Matrix Proteins
• Fplc
• Guanidine
• Hydrochloric Acid
• Integrin-Binding Sialoprotein
• Ion-exchange Chromatography
• Mice
• N-linked Glycoprotein
• Non-collagenous Protein
• Osteopontin
• Phosphoproteins
• Sialoglycoproteins
• Small Integrin-binding Ligand
• Sodium Chloride
• Stains-all Staining
• Ultrafiltration

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-9012-2_21

start page

• 211

end page

• 218

volume

• 1922

URL

• http://dx.doi.org/10.1007/978-1-4939-9012-2_21