Whole-Mount In Situ Hybridization of Mouse Embryos Using DIG-Labeled RNA Probes. Academic Article

abstract

• Whole-mount in situ hybridization (WMISH) is a commonly used technique for visualizing the expression profile of mRNAs in embryos. Unlike traditional in situ hybridization techniques, which require thin tissue sections, the WMISH technique allows gene expression patterns to be assessed over the entire embryo and structure. Here, we describe the detailed procedural steps of WMISH, including probe production, embryo fixation and staining, and post-hybridization signal detection. Using this protocol, we visualized highly specific expression patterns of Sonic hedgehog and Bmp4 mRNAs in E12.5 mouse embryos.

authors

• Wang, Xiaofang

published proceedings

• Methods Mol Biol

author list (cited authors)

• Wu, J., & Wang, X.

citation count

• 4

complete list of authors

• Wu, Jingyi||Wang, Xiaofang

publication date

• January 2019

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Bone Morphogenetic Protein 4
• Craniofacial Development
• Digoxigenin
• Digoxigenin Labeling
• Embryo, Mammalian
• Embryonic Development
• Gene Expression
• Gene Expression Regulation, Developmental
• Hedgehog Proteins
• In Situ Hybridization
• Mice
• Mouse Embryo
• RNA Probes
• RNA, Messenger
• Rna Probe
• Rnase-free
• Whole-mount

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-9012-2_15

start page

• 151

end page

• 159

volume

• 1922

URL

• http://dx.doi.org/10.1007/978-1-4939-9012-2_15