In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes. Academic Article uri icon

abstract

  • In situ hybridization is a commonly used technique using an antisense RNA probe to localize a specific RNA sequence on histological sections. This approach can visualize the expression pattern of a gene of interest in a portion of tissues. Here, we detail an optimized method for performing in situ hybridization on mouse paraffin sections using digoxigenin (DIG)-labeled RNA probes.

published proceedings

  • Methods Mol Biol

author list (cited authors)

  • Wu, J., Feng, J. Q., & Wang, X

citation count

  • 3

complete list of authors

  • Wu, Jingyi||Feng, Jian Q||Wang, Xiaofang

publication date

  • March 2019