In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes
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In situ hybridization is a commonly used technique using an antisense RNA probe to localize a specific RNA sequence on histological sections. This approach can visualize the expression pattern of a gene of interest in a portion of tissues. Here, we detail an optimized method for performing in situ hybridization on mouse paraffin sections using digoxigenin (DIG)-labeled RNA probes.
author list (cited authors)
Wu, J., Feng, J. Q., & Wang, X.