In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes. Academic Article

abstract

• In situ hybridization is a commonly used technique using an antisense RNA probe to localize a specific RNA sequence on histological sections. This approach can visualize the expression pattern of a gene of interest in a portion of tissues. Here, we detail an optimized method for performing in situ hybridization on mouse paraffin sections using digoxigenin (DIG)-labeled RNA probes.

authors

• Feng, Jian
• Wang, Xiaofang

published proceedings

• Methods Mol Biol

author list (cited authors)

• Wu, J., Feng, J. Q., & Wang, X.

citation count

• 4

complete list of authors

• Wu, Jingyi||Feng, Jian Q||Wang, Xiaofang

publication date

• January 2019

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Digoxigenin
• Digoxigenin Labeling
• Embryo, Mammalian
• Gene Expression
• Gene Expression Regulation, Developmental
• In Situ Hybridization
• MRNA
• Mice
• Paraffin Embedding
• Paraffin Section
• RNA Probes
• RNA, Messenger
• Rna Probe
• Rnase-free

PubMed Central ID

• 30838574

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-9012-2_16

start page

• 163

end page

• 171

volume

• 1922

URL

• http://dx.doi.org/10.1007/978-1-4939-9012-2_16