In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes Academic Article uri icon

abstract

  • In situ hybridization is a commonly used technique using an antisense RNA probe to localize a specific RNA sequence on histological sections. This approach can visualize the expression pattern of a gene of interest in a portion of tissues. Here, we detail an optimized method for performing in situ hybridization on mouse paraffin sections using digoxigenin (DIG)-labeled RNA probes.

author list (cited authors)

  • Wu, J., Feng, J. Q., & Wang, X.

citation count

  • 2

publication date

  • March 2019