Induction and characterization of acrosome reaction in equine spermatozoa.

abstract

• Equine spermatozoa were incubated in a chemically defined medium for 8 hours. The medium preserved spermatozoal viability, as assessed by total spermatozoal motility, progressive spermatozoal motility, and spermatozoal exclusion of eosin stain. Effects of time and divalent cation ionophore, A23187, on the occurrence and character of the spermatozoal acrosome reaction were determined. Two light microscopic assays, a triple-stain technique and a chlortetracycline fluorescence assay, were calibrated with transmission electron microscopy for detection of the acrosome reaction. Incubation time and A23187 addition increased the percentage of acrosome reactions in sperm populations (P less than 0.05).

authors

• Varner, Dickson

published proceedings

• Am J Vet Res

author list (cited authors)

• Varner, D. D., Ward, C. R., Storey, B. T., & Kenney, R. M.

citation count

• 69

complete list of authors

• Varner, DD||Ward, CR||Storey, BT||Kenney, RM

publication date

• September 1987

published in

• American Journal of Veterinary Research  Journal

keywords

• Acrosome
• Animals
• Calcimycin
• Horses
• Male
• Sperm Capacitation
• Sperm Motility
• Spermatozoa

PubMed Central ID

• 3116891

start page

• 1383

end page

• 1389

volume

• 48

issue

• 9