Analysis of Photothermal Release of Oligonucleotides from Hollow Gold Nanospheres by Surface-Enhanced Raman Scattering Academic Article uri icon


  • 2016 American Chemical Society. The photothermal release of single-stranded DNA (ssDNA) from the surface of gold nanoparticles of different shapes and sizes is a promising mode of delivering DNA for gene-therapy applications. Here, we demonstrate the first targeted photothermal release of ssDNA from hollow gold nanospheres (HGNs) and analyze the release of the ssDNA using quantitative surface-enhanced Raman scattering (SERS). The HGNs used demonstrate a tunable localized surface plasmon resonance (LSPR) frequency while maintaining size consistency, allowing for selective ssDNA release based on matching the excitation frequency to the plasmon resonance. It is shown that HGNs with resonances at 760 and 670 nm release significant amounts of ssDNA when excited via 785 and 640 nm lasers, respectively. When excited with a wavelength far from the LSPR of the particles, the ssDNA release is negligible. This is the first demonstration of SERS to analyze the amount of ssDNA photothermally released from the surface of HGNs. In contrast to traditional fluorescence measurements, this SERS-based approach provides quantitatively robust data for analysis of ssDNA release and lays a strong foundation for future studies exploiting plasmonically induced ssDNA release.

published proceedings


altmetric score

  • 5.15

author list (cited authors)

  • Mackanic, D. G., Mabbott, S., Faulds, K., & Graham, D.

citation count

  • 7

complete list of authors

  • Mackanic, David G||Mabbott, Samuel||Faulds, Karen||Graham, Duncan

publication date

  • September 2016