Differential Mechanism of Action of 3,4',7-O-trimethylquercetin in Three Types of Ovarian Cancer Cells. Academic Article uri icon


  • BACKGROUND/AIM: 3,4',7-O-trimethylquercetin (34'7TMQ), a derivative of quercetin, inhibited ovarian cancer cell migration and invasion without affecting proliferation. In this study, the apoptotic effect of 34'7TMQ on three cancer cell lines (CRL-1978, CRL-11731, SK-OV-3) was evaluated. MATERIALS AND METHODS: Expression of pro-apoptotic proteins such as Bax/Bcl-2 ratio, p38 MAP kinase, and caspase-9 were measured by western blot analysis. Annexin-V staining was performed to visualize apoptotic signaling. RESULTS: Caspase-9 was up-regulated in all three cell lines. Bax/Bcl-2 ratio was up-regulated in CRL-1978 and SK-OV-3 but down-regulated in CRL-11731. The p38 MAPK was down-regulated in CRL-1978, up-regulated in SK-OV-3, and had differential expression in CRL-11731. Annexin V staining indicated that 34'7TMQ at 6.25 M induced apoptotic signaling in the CRL-1978 ovarian cancer cell line. CONCLUSION: 34'7TMQ induced apoptosis in three types of cancer cell lines but it appears to have a different mechanism of action in each cell line.

published proceedings

  • Anticancer Res

altmetric score

  • 0.5

author list (cited authors)

  • Ashraf, A., Afroze, S. H., Yamauchi, K., Zawieja, D. C., Keuhl, T. J., Erlandson, L. W., & Uddin, M. N.

citation count

  • 5

complete list of authors

  • Ashraf, AHM Zuberi||Afroze, Syeda H||Yamauchi, Kosei||Zawieja, David C||Keuhl, Thomas J||Erlandson, Laura Weiser||Uddin, Mohammad N

publication date

  • September 2018