Draft genome sequences of five recent human uropathogenic Escherichia coli isolates. Academic Article

abstract

• This study reports the release of draft genome sequences of five isolates of uropathogenic Escherichia coli (UPEC), isolated from patients suffering from uncomplicated cystitis in 2012 in Ann Arbor, Michigan. Phylogenetic analyses revealed that these strains belonged to E. coli phylogroups B2 and D and are closely related to known UPEC strains. Comparative genomic analysis revealed that more conserved proteins were shared between these recent isolates and UPEC strains causing cystitis than those causing pyelonephritis. Additional genomic comparisons identified that three isolates encode a type III secretion system (T3SS) and a putative T3SS effector gene cluster along with an invasin-like outer membrane protein. The presence of T3SS genes is a rare occurrence among UPEC strains. These genomes further substantiate the heterogeneity of the gene pool of UPEC and provide a foundation for comparative genomic studies using recent clinical isolates.

authors

• Subashchandrabose, Sargurunathan

published proceedings

• Pathog Dis

altmetric score

• 1

author list (cited authors)

• Subashchandrabose, S., Hazen, T. H., Rasko, D. A., & Mobley, H.

citation count

• 16

complete list of authors

• Subashchandrabose, Sargurunathan||Hazen, Tracy H||Rasko, David A||Mobley, Harry LT

publication date

• October 2013

publisher

• Oxford University Press (OUP)  Publisher

keywords

• Cystitis
• DNA, Bacterial
• Draft Genomes
• Escherichia Coli Infections
• Escherichia Coli Proteins
• Genome, Bacterial
• Genotype
• Humans
• Michigan
• Phylogeny
• Sequence Analysis, DNA
• Type Iii Secretion System
• Uropathogenic Escherichia Coli
• Virulence Factors

Digital Object Identifier (DOI)

• 10.1111/2049-632X.12059

start page

• 66

end page

• 70

volume

• 69

issue

• 1

URL

• http://dx.doi.org/10.1111/2049-632x.12059