Roles of DMP1 processing in osteogenesis, dentinogenesis and chondrogenesis. Academic Article uri icon


  • Dentin matrix protein 1 (DMP1) is an acidic protein that plays critical roles in osteogenesis and dentinogenesis. Protein chemistry studies have demonstrated that DMP1 primarily exists as processed NH and COOH-terminal fragments in the extracellular matrix of bone and dentin. Our earlier work showed that the substitution of Asp (a residue at a cleavage site) by Ala blocks the processing of mouse DMP1 in vitro. Recently, we generated transgenic mice expressing this mutant DMP1 (designated 'D213A-DMP1'). By crossbreeding these transgenic mice with Dmp1-knockout (Dmp1-KO) mice, we obtained mice expressing the D213A-DMP1 transgene in the Dmp1-null background (named 'Dmp1-KO/D213A-Tg' mice). In this study, we analyzed the long bone, mandible, dentin, and cartilage of Dmp1-KO/D213A-Tg mice in comparison with wild-type, Dmp1-KO, and Dmp1-KO mice expressing the normal DMP1 transgene (Dmp1-KO/normal-Tg). Our results showed that D213A-DMP1 was barely cleaved in the dentin matrix of Dmp1-KO/D213A-Tg mice and the expression of D213A-DMP1 failed to rescue the developmental defects in Dmp1-null mice. Interestingly, enlarged growth plates and condylar cartilages were observed in Dmp1-KO/D213A-Tg mice, indicating a potential role of the full-length form of DMP1 in chondrogenesis.

published proceedings

  • Cells Tissues Organs

author list (cited authors)

  • Sun, Y., Chen, L. i., Ma, S. u., Zhou, J., Zhang, H., Feng, J. Q., & Qin, C.

citation count

  • 24

complete list of authors

  • Sun, Yao||Chen, Li||Ma, Su||Zhou, Jin||Zhang, Hua||Feng, Jian Q||Qin, Chunlin

publication date

  • August 2011