Exploring key orientations at protein-protein interfaces with small molecule probes.
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Small molecule probes that selectively perturb protein-protein interactions (PPIs) are pivotal to biomedical science, but their discovery is challenging. We hypothesized that conformational resemblance of semirigid scaffolds expressing amino acid side-chains to PPI-interface regions could guide this process. Consequently, a data mining algorithm was developed to sample huge numbers of PPIs to find ones that match preferred conformers of a selected semirigid scaffold. Conformations of one such chemotype (1aaa; all methyl side-chains) matched several biomedically significant PPIs, including the dimerization interface of HIV-1 protease. On the basis of these observations, four molecules 1 with side-chains corresponding to the matching HIV-1 dimerization interface regions were prepared; all four inhibited HIV-1 protease via perturbation of dimerization. These data indicate this approach may inspire design of small molecule interface probes to perturb PPIs.
author list (cited authors)
Ko, E., Raghuraman, A., Perez, L. M., Ioerger, T. R., & Burgess, K.
complete list of authors
Ko, Eunhwa||Raghuraman, Arjun||Perez, Lisa M||Ioerger, Thomas R||Burgess, Kevin