Simultaneous determination of Acteoside, Astragaloside IV and Icariside-I in the traditional Chinese medicinal preparation Shenbao by HPLC-MS uri icon

abstract

  • A liquid chromatography-electrospray (ES)-mass spectrometric method for the simultaneous determination of Acteoside, Astragaloside IV and Icariside-I in the Traditional Chinese Medicinal Preparation Shenbao tablets is described. The samples were separated on an Alltima C18 column by linear gradient elution using water-acetonitrile as the mobile phase. Some operational parameters of the ESI interface were optimized. The method is linear over the range of 0.1-10g mL-1 for Acteoside, Astragaloside IV, and 0.03-3g mL-1 for Icariside-I. The method has a precision (%CV) of <20%, and an accuracy (%RE) of < 10%. It can be used as a complementary method for quality control of Shenbao Tablets while HPLC-UV can be used for the other main components (Icariin, Icariside-II, Psoralen, Isopsoralen, and Osthol). 2006 Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH.

published proceedings

  • CHROMATOGRAPHIA

author list (cited authors)

  • Tu, Y., Zhao, L., Zhu, L., Wang, G., & Xiang, B.

citation count

  • 5

complete list of authors

  • Tu, Ying||Zhao, Luhua||Zhu, Lin||Wang, Guangji||Xiang, Bingren

publication date

  • October 2006