Detection of the ergosterol and episterol isomers lichesterol and fecosterol in nystatin-resistant mutants of Neurospora crassa.

abstract

• Wild-type Neurospora crassa is completely inhibited by 5 ppm nystatin. Ultraviolet-induced mutants have been isolated that grow in the presence of 60 ppm of the antibiotic. Gas-liquid chromatographic, mass spectroscopic, and nuclear magnetic resonance analyses showed the wild-type sterols to be ergosterol (ergosta-5,7,22-trien-3-ol) and episterol (ergosta-7,24(28)-dien-3-ol) in a 3:1 ratio. The mutants contained lichesterol (ergosta-5,8,22-trien-3-ol) and fecosterol (ergosta-8,24(28)-dien-3-ol) in a 2:1 ratio, differing from the wild type only in the position of the B-ring unsaturation. A deficiency of an ergosta-8,24 (28)-dien-3-ol:ergosta-7,24(28)-dien-3-ol isomerase is indicated. 1974 Plenum Publishing Corporation.

authors

• Safe, Stephen

published proceedings

• Biochem Genet

altmetric score

• 3

author list (cited authors)

• Morris, D. C., Safe, S., & Subden, R. E.

citation count

• 18

complete list of authors

• Morris, DC||Safe, S||Subden, RE

publication date

• December 1974

publisher

• Springer Nature  Publisher

published in

• Biochemical Genetics  Journal

keywords

• Chromatography, Gas
• Drug Resistance, Microbial
• Ergosterol
• Isomerism
• Mass Spectrometry
• Mutation
• Neurospora
• Neurospora Crassa
• Nystatin
• Phytosterols

Digital Object Identifier (DOI)

• 10.1007/BF00486063

start page

• 459

end page

• 466

volume

• 12

issue

• 6

URL

• http://dx.doi.org/10.1007/bf00486063