Betulinic acid attenuates dexamethasone-induced oxidative damage through the JNK-P38 MAPK signaling pathway in mice. Academic Article uri icon


  • Dexamethasone (Dex), a potent anti-inflammatory/immunosuppressive agent, has been shown to induce oxidative stress. Betulinic acid (BA) is a pentacyclic lupane triterpene with a potent antioxidant activity. The aim of this study was to investigate the ameliorative effect and underlying mechanisms of BA on Dex-induced oxidative damage. Mice were pretreated with BA orally (0, 0.25, 0.5, and 1.0mg/kg) daily for 14 days, and then a single dose of Dex (25mg/kg body weight) was administered intraperitoneally 8h after the last administration of BA to induce oxidative stress. BA pretreatment significantly alleviated Dex-induced changes of blood biochemical indices, increased the total antioxidant capacity (T-AOC), the activity of superoxide dismutase (SOD), and the ability of inhibiting hydroxyl radical (AIHR), reduced the level of malondialdehyde (MDA) in serum. Moreover, BA pretreatment enhanced the T-AOC, AIHR and the activity of peroxidase (POD) in liver, spleen and thymus. Concomitant with these biochemical parameters, BA pretreatment significantly reduced gene and protein expressions of apoptosis signal-regulating kinase 1 (ASK1), c-Jun N-terminal kinase (JNK) and P38 mitogen-activated protein kinase (P38 MAPK) in the lymphatic organs of Dex-treated mice. BA was found to effectively attenuate Dex-induced oxidative damage. These protective effects may be mediated in part through the JNK-P38 MAPK signaling transduction pathway and BA may be a potential therapeutic agent due to its anti-oxidative properties.

published proceedings

  • Biomed Pharmacother

author list (cited authors)

  • Zhu, L., Yi, X., Zhao, J., Yuan, Z., Wen, L., Pozniak, B., ... Yi, J.

citation count

  • 27

complete list of authors

  • Zhu, Lijuan||Yi, Xianglian||Zhao, Jing||Yuan, Zhihang||Wen, Lixin||Pozniak, Blazej||Obminska-Mrukowicz, Bozena||Tian, Yanan||Tan, Zhuliang||Wu, Jing||Yi, Jine

publication date

  • January 2018