Physiology and proteomics of the water-deficit stress response in three contrasting peanut genotypes. Academic Article uri icon

abstract

  • Peanut genotypes from the US mini-core collection were analysed for changes in leaf proteins during reproductive stage growth under water-deficit stress. One- and two-dimensional gel electrophoresis (1- and 2-DGE) was performed on soluble protein extracts of selected tolerant and susceptible genotypes. A total of 102 protein bands/spots were analysed by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) analysis. Forty-nine non-redundant proteins were identified, implicating a variety of stress response mechanisms in peanut. Lipoxygenase and 1l-myo-inositol-1-phosphate synthase, which aid in inter- and intracellular stress signalling, were more abundant in tolerant genotypes under water-deficit stress. Acetyl-CoA carboxylase, a key enzyme of lipid biosynthesis, increased in relative abundance along with a corresponding increase in epicuticular wax content in the tolerant genotype, suggesting an additional mechanism for water conservation and stress tolerance. Additionally, there was a marked decrease in the abundance of several photosynthetic proteins in the tolerant genotype, along with a concomitant decrease in net photosynthesis in response to water-deficit stress. Differential regulation of leaf proteins involved in a variety of cellular functions (e.g. cell wall strengthening, signal transduction, energy metabolism, cellular detoxification and gene regulation) indicates that these molecules could affect the molecular mechanism of water-deficit stress tolerance in peanut.

published proceedings

  • Plant Cell Environ

author list (cited authors)

  • Kottapalli, K. R., Rakwal, R., Shibato, J., Burow, G., Tissue, D., Burke, J., ... Payton, P.

citation count

  • 110

complete list of authors

  • Kottapalli, Kameswara Rao||Rakwal, Randeep||Shibato, Junko||Burow, Gloria||Tissue, David||Burke, John||Puppala, Naveen||Burow, Mark||Payton, Paxton

publication date

  • April 2009

publisher