[Expression of glycoprotein hepatitis C virus in mammalian cell and application of purified protein for detection of antibody against E2 in hepatitis C patients].
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OBJECTIVE: E2 glycoprotein of hepatitis C virus was expressed in mammalian cell and purified for detection of antibody against E2 in hepatitis C patients. METHODS: E2/NS1 gene derived from HCV was inserted into expression vector containing six His tag. The recombinant plasmid was transfected into mammalian cells to express E2 glycoprotein expression. E2 glycoprotein was purified by affinity chromophotography. The purified protein was used to establish EIA method for detection of antibodies against E2 in hepatitis C patients. RESULT: Expressed E2 glycoprotein was 7.0 x 10(4). Purification of the purified E2 protein was 90.2%. Twenty-nine patients were anti-E2 antibody positive(82.9%). CONCLUSION: It was the first time to establish EIA method for detection of anti-E2 antibody by purified E2 glycoprotein in China. E2 glycoprotein expressed in mammalian cells had good immunogenity and could increase the sensibility of anti-HCV detection. It suggests that E2 glycoprotein may be useful for development of new anti-HCV reagents.
