Antibody response to E2 glycoprotein induced in mice by immunization with plasmid DNA containing sequence derived from a Chinese genotype III/2a isolate of hepatitis C virus. uri icon

abstract

  • OBJECTIVE: To induce antibody response against E2 glycoprotein derived from a Chinese genotype III/2a isolate of hepatitis C virus (HCV) in BALB/C mice by plasmid DNA-based immunization. METHODS: Plasmid p3W14 containing E2/NS1 gene derived from a Chinese genotype III/2a isolate of HCV, which was verified to express 70 kDa E2 glycoprotein in NIH3T3 cells, was used for direct intramuscular injection in BALB/C mice. Specific antibody to E2 glycoprotein was detected by recombinant E2 protein. RESULTS: Specific anti-E2 antibody could be detected in mice inoculated with p3W14(5/6). Titer of antibody ranged from 1:15 to 1:120. CONCLUSION: Successfully inducing anti-E2 antibody in mice by plasmid DNA-based immunization containing E2/NS1 gene from a Chinese genotype III/2a isolate of HCV is the first time in China.

published proceedings

  • Chin Med J (Engl)

author list (cited authors)

  • Wu, C., Tao, Q., & Feng, B.

citation count

  • 0

complete list of authors

  • Wu, C||Tao, Q||Feng, B

publication date

  • February 1999