Phenotypic variation during micropropagation of the chimeralRhododendron President Roosevelt Academic Article

abstract

• The putative periclinal chimera Rhododendron x limbatum 'President Roosevelt' was used to study the origin of shoots in vitro. Genotypic segregation readily occurred in vitro. Numerous phenotypes were observed, although most shoots were either entirely green or maintained the original variegation pattern. Derivatives of the third apical layer were rarely involved in shoot formation. A reversed chimeral form was isolated. Adventitious shoots were usually miniaturized and rapidly proliferating, but axillary shoots had thicker stems, larger leaves and proliferated more slowly. Corolla tissue produced stunted, leafy shoots; no variegated shoots were produced from floret explants. In shoot tip cultures the addition of 40 M 2iP without IBA resulted in the greatest number of shoots. Explant choice was the most critical factor for maintenance of foliar variegation. 1990 Kluwer Academic Publishers.

authors

• Lineberger, Dan

published proceedings

• Plant Cell, Tissue and Organ Culture (PCTOC)

author list (cited authors)

• Pogany, M. F., & Lineberger, R. D.

citation count

• 7

complete list of authors

• Pogany, Mary F||Lineberger, R Daniel

publication date

• January 1990

publisher

• Springer Nature  Publisher

published in

• Plant Cell, Tissue and Organ Culture: an international journal on in vitro culture of higher plants  Journal

Digital Object Identifier (DOI)

• 10.1007/bf00047612

start page

• 201

end page

• 209

volume

• 21

issue

• 3

URL

• http://dx.doi.org/10.1007/bf00047612