Somaclonal and radiation induced variation in Lycopersicon esculentum
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Tissue culture and ionizing radiation were used to induce or to allow the expression of phenotypic variation in several processing tomato lines. Cotyledon cultures of OH7870 and OH832 were exposed to 0, 1500, 3000, 4500 and 6500 rads of thermal neutron radiation. Plants regenerated from these cultures were grown in a field planting to observe variation. A strong suppression of growth in vitro was observed at the 1500 rad level, while growth in treatments exposed to more than 1500 rads was directly correlated to radiation dose. Intermediate levels of radiation dosage led to the greatest number of observable variants. Seedling control populations exhibited the least variation. Progency evaluation showed segregation, indicating some variation was due to genetic changes. © 1989.
author list (cited authors)
Montagno, T. J., Lineberger, R. D., & Berry, S. Z.