Artificial feeding system for the squash bug, Anasa tristis (De Geer) (Heteroptera: Coreidae)
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Squash bugs, Anasa tristis (De Geer) (Heteroptera: Coreidae), did not feed on liquid diet-filled feeding sachets used traditionally for feeding homopterans, or on pouch-like artificial feeding source (AFS) containing a meridic diet developed for rearing the western tarnished plant bug, Lygus hesperus Knight, another heteropteran. However, excised cubes of squash fruit, vacuum infiltrated with a suspension of the desired diet and offered above a screen barrier, were accepted. During a 48-hr testing period, all cube-fed adult insects survived, while 35% of those fed on the meridic diet offered in parafilm pouches and 40% of those fed on 5% sucrose offered in sachets died. After blue food coloring was added to the diets as a marker, the excretory fluids of 75% of insects fed on infiltrated cubes was blue in color indicating that feeding had occurred, while no blue defecation occurred when dye-amended diets were offered in the other two systems. The A. tristis AFS provides a convenient system for studying the feeding behavior of squash bugs and provides an alternative to whole plants for studying the etiology of cucurbit yellow vine disease.
author list (cited authors)
Bextine, B. R., Wayadande, A., Bruton, B. D., Pair, S. D., Mitchell, F., & Fletcher, J.
complete list of authors
Bextine, BR||Wayadande, A||Bruton, BD||Pair, SD||Mitchell, F||Fletcher, J