Simulatenous analyses of cocaine and its metabolite benzoylecgonine in urine by LC-MS/MS Academic Article uri icon


  • Objective: To establish a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous analysis of cocaine (COC) and its metabolite benzoylecgonine(BZE) in urine samples. Methods: A single dose of COC (10 mg/kg) was injected intraperitoneally into guinea pigs and urine samples were collected for 7 days. The urine samples were extracted by auto solid phase extraction (SPE), separated by the Allure PFP propyl column with a mobile phase consisting of methanol and 20 mmol/L ammonium acetate buffer [0.1% formic acid (80:20, V/V)], and then analyzed by LC-MS/MS. Multiple reaction monitoring (MRM) mode was used to analyze COC(m/z 304.2182.3, m/z 304.2150.1) and BZE (m/z 290.2168.3, m/z 290.2105.0). Results: COC and BZE showed a fairly good linearity over the range of 2.0-100 ng/mL (r=0.999 5). The detection limit was 0.5 ng/mL. The recovery rate was greater than 90% and the deviation of intra- and inter-day precision was less than 6%. BZE was the major target detected in urine samples, and its detection window was longer than COC. Conclusion: This newly developed method shows high sensitivity and selectivity, and is suitable for the simultaneous analysis of cocaine and benzoylecgonine in urine samples.

published proceedings

  • Journal of Forensic Medicine

author list (cited authors)

  • Sun, Q. R., Xiang, P., Yan, H., & Shen, M.

complete list of authors

  • Sun, QR||Xiang, P||Yan, H||Shen, M

publication date

  • August 2008