A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-linear Gradients. Chapter

abstract

• Microfluidic technology allows fast and precise measurement of chemotaxis responses to both attractant and repellent signals. One of the major drawbacks of current microfluidic chemotaxis assays is the presence of bacterial cells within the concentration gradient flow field, which has the potential for flow effects masking the chemotaxis response. This chapter describes a new microfluidic device for producing stable concentration gradients and measuring the response of cells to the gradient without exposing them to any flow. Unlike other methods described in the literature, this method is capable of producing gradients of any shape, almost instantaneously, allowing the measurement of time-dependent response of cells to a variety of signals.

authors

• Jayaraman, Arul

author list (cited authors)

• Sule, N., Penarete-Acosta, D., Englert, D. L., & Jayaraman, A.

citation count

• 1

complete list of authors

• Sule, Nitesh||Penarete-Acosta, Daniel||Englert, Derek L||Jayaraman, Arul

Book Title

• BACTERIAL CHEMOSENSING

publication date

• January 2018

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Behavioral Assay
• Chemotaxis
• Equipment Design
• Escherichia Coli
• Lab-On-A-Chip Devices
• Microfluidic Analytical Techniques
• Microfluidic Device
• Microscopy, Fluorescence

PubMed Central ID

• 29429081

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-7577-8_5

International Standard Book Number (ISBN) 13

• 978-1-4939-7576-1

start page

• 47

end page

• 59

volume

• 1729

URL

• http://dx.doi.org/10.1007/978-1-4939-7577-8_5