Effect of tropisetron (trop) and splanchnic (spln) and vagal (vag) denervations on intake of an imbalanced amino acid diet (IMB)
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Rats given an 1MB show attenuated food intake (FI) within 3h; 5HT, receptors and VAG and SPLN nerves are involved in this response. This study had 7 groups (grps, n=10-16): VENT, ventral vagal branch cut above the hepatic vagal branch (HVB) + bilateral SPLN cuts; VENT-B, ventral branch cut below the HVB + SPLN cuts; VENT-B; and sham operation (S). Diets were milk (3d), casein gel (5d), and low protein basal (8d). The last 2d on the basal diet all rats were injected with saline (SAL) starting at lights out; FI was recorded at 3, 6, 12, and 24h intervals. Next the grps were injected with either TROP (9mg/kg) or SAL, except for the VENT-B grp which received only SAL. An 1MB was given with interval measurements on dl and daily measurements for 7d. By 24h TROP had increased the grps' FI over SAL grps. However, the VENT+SPLN-TROP grps' FI was less than the S-TROP and VENT-B+SPLN-TROP grps, which suggest the HVB is important in the TROP response. By day 7 the two VENT-SPLN grps had the highest body weight (BW, as % of starting BW), suggesting that loss of the HVB enhances BW gain on an 1MB. BW in VENT-B + SPLN equalled VENT-B so loss of SPLN didn't affect recovery. Supported by BCD Res. Funds & DK42274 to D.W.G.
