Binding of collagen to Staphylococcus aureus Cowan 1. Academic Article uri icon


  • Collagen binds to a receptor protein present on the surfaces of Staphylococcus aureus cells. Binding of 125I-labeled type II collagen to its bacterial receptor is reversible, and Scatchard plot analysis indicates the presence of one class of receptor that occurs on an average of 3 X 10(4) copies per cell and binds type II collagen with a Kd of 10(-7) M. Studies on the specificity of collagen cell binding indicate that the receptor does not recognize noncollagenous proteins but binds all of the different collagen types tested (types I to VI). Furthermore, isolated collagen alpha chains and peptides generated by cyanogen bromide cleavage of type I collagen alpha chains are recognized by the receptor as indicated by the ability of these polypeptides to inhibit binding of 125I-labeled type II collagen to staphylococcal cells. Synthetic collagen analogs were tested as inhibitors of type II collagen binding to bacterial cells. The peptides (Pro-Gly-Pro)n, (Pro-Pro-Gly)10, and (Pro-OH-Pro-Gly)10 were recognized by the receptor, whereas the peptides (Pro-Ala-Gly)n and polyproline showed no inhibitory activity.

published proceedings

  • J Bacteriol

altmetric score

  • 3

author list (cited authors)

  • Speziale, P., Raucci, G., Visai, L., Switalski, L. M., Timpl, R., & Hk, M.

citation count

  • 125

complete list of authors

  • Speziale, P||Raucci, G||Visai, L||Switalski, LM||Timpl, R||Höök, M

publication date

  • July 1986