Isolation and characterization of heparin from human mastocytoma tissue. Academic Article uri icon

abstract

  • Polysaccharide was isolated from human spleen mastocytoma by proteolytic digestion, precipitation with cetylpyridinium chloride, digestion with chondroitinase ABC, and ion-exchange chromatography on DEAE-cellulose. The final product (0.7 mg per g of starting material, MW 8000) behaved like standard heparin on ion-exchange chromatography and on electrophoresis, and contained D-glucuronic acid, L-iduronic acid, D-glucosamine and sulfate in the proportions expected for heparin. Affinity chromatography on antithrombin-Sepharose separated a distinct high-affinity fraction (4-5% of the total material). Structural analysis of this fraction showed that about 10% of the D-glucosamine residues were N-acetylated, the remainder N-sulfated. The anticoagulant activity of the isolated heparin was 71 B.P. units per mg (whole-blood system), or 30 units per mg (anti-thrombin and chromogenic substrate). 205 and 10-15 units per mg (chromogenic assay) were found for high and low affinity fractions, respectively. These results demonstrate conclusively the occurrence of heparin in a human tissue.

published proceedings

  • Thromb Haemost

author list (cited authors)

  • Thunberg, L., Hk, M., Lindahl, U., Abildgaard, U., & Langholm, R.

citation count

  • 8

complete list of authors

  • Thunberg, L||Höök, M||Lindahl, U||Abildgaard, U||Langholm, R

publication date

  • January 1980

publisher