Biosynthesis of heparin. Loss of C-5 hydrogen during conversion of d-glucuronic to l-iduronic acid residues Academic Article

abstract

• Microsomal fraction from mouse mastocytoma was incubated with UDP-N-acetylglucosamine and 3H-, 14C-labeled UDP-glucuronic acid. Enzymatic sulfation of the resulting heparin-precursor polysaccharide (by addition of 3-phosphoadenylylsulfate) was accompanied by selective loss of 3H from C5, but not from C2 or C4, of the uronic acid residues. Analysis of the sulfated product showed C5-3H in the glucuronic acid but not in the iduronic acid component. The formation of l-iduronic acid residues by C5-epimerization of d-glucuronic acid units at the polymer level thus involves loss of hydrogen at C5. 1976.

authors

• Hook, Magnus

published proceedings

• Biochemical and Biophysical Research Communications

author list (cited authors)

• Lindahl, U., Jacobsson, I., Hk, M., Backstrm, G., & Feingold, D. S.

citation count

• 48

complete list of authors

• Lindahl, Ulf||Jacobsson, Ingvar||Hőők, Magnus||Backstrőm, Gudrun||Feingold, David S

publication date

• May 1976

publisher

• Elsevier BV  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

Digital Object Identifier (DOI)

• 10.1016/0006-291x(76)91073-1

start page

• 492

end page

• 499

volume

• 70

issue

• 2

URL

• http://dx.doi.org/10.1016/0006-291x(76)91073-1