Biosynthesis of heparin. Loss of C-5 hydrogen during conversion of d-glucuronic to l-iduronic acid residues Academic Article uri icon


  • Microsomal fraction from mouse mastocytoma was incubated with UDP-N-acetylglucosamine and 3H-, 14C-labeled UDP-glucuronic acid. Enzymatic sulfation of the resulting heparin-precursor polysaccharide (by addition of 3-phosphoadenylylsulfate) was accompanied by selective loss of 3H from C5, but not from C2 or C4, of the uronic acid residues. Analysis of the sulfated product showed C5-3H in the glucuronic acid but not in the iduronic acid component. The formation of l-iduronic acid residues by C5-epimerization of d-glucuronic acid units at the polymer level thus involves loss of hydrogen at C5. 1976.

published proceedings

  • Biochemical and Biophysical Research Communications

author list (cited authors)

  • Lindahl, U., Jacobsson, I., Hk, M., Backstrm, G., & Feingold, D. S.

citation count

  • 48

complete list of authors

  • Lindahl, Ulf||Jacobsson, Ingvar||Hőők, Magnus||Backstrőm, Gudrun||Feingold, David S

publication date

  • May 1976