Binding of heparin and heparan sulphate to rat liver cells. Academic Article

abstract

• The binding of pig mucosal heparin and rat liver heparan sulphate to rat liver cells is demonstrated. The process is shown to be time dependent, reversible and saturable. The maximal amount of heparin bound to the cells exceeds that of heparan sulphate, on a molar basis. The binding of both polysaccharides is specific, in that excess amounts of glycosaminoglycans other than heparin-related do not affect the binding reaction. The binding of heparin to cells was markedly reduced when incubations were performed at low temperature or after trypsin treatment of the cells. 1977.

authors

• Hook, Magnus

published proceedings

• Biochem Biophys Res Commun

altmetric score

• 6

author list (cited authors)

• Kjelln, L., Oldberg, A., Rubin, K., & Hk, M.

citation count

• 108

complete list of authors

• Kjellén, L||Oldberg, A||Rubin, K||Höök, M

publication date

• January 1977

publisher

• Elsevier  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

keywords

• Animals
• Binding Sites
• Binding, Competitive
• Glycosaminoglycans
• Heparin
• Heparitin Sulfate
• In Vitro Techniques
• Kinetics
• Ligands
• Liver
• Rats

Digital Object Identifier (DOI)

• 10.1016/0006-291x(77)91384-5

start page

• 126

end page

• 133

volume

• 74

issue

• 1

URL

• http://dx.doi.org/10.1016/0006-291x(77)91384-5