Histone-DNA interactions within chromatin. Isolation of histones from DNA-histone adducts induced in nuclei by UV light. Academic Article

abstract

• We have developed a method by which to isolate histones that have been crosslinked to DNA following irradiation of calf thymus nuclei by UV light. The procedure involves separation of protein-DNA adducts from uncrosslinked protein by Sepharose 4B chromatography under dissociating conditions. Histones which are crosslinked to DNA are released by chemical hydrolysis of the DNA and identified by SDS gel electrophoresis. The results indicate that, of the histones, H1 and H3 become crosslinked to the DNA most readily under our irradiation conditions.

authors

• Kunkel, Gary

published proceedings

• Nucleic Acids Res

author list (cited authors)

• Kunkel, G. R., & Martinson, H. G

citation count

• 31

complete list of authors

• Kunkel, GR||Martinson, HG

publication date

• November 1978

publisher

• Oxford University Press (OUP)  Publisher

published in

• Nucleic Acids Research (NAR)  Journal

keywords

• Animals
• Cattle
• Cell Nucleus
• Chromatin
• DNA
• Histones
• Molecular Weight
• Protein Binding
• Thymus Gland
• Ultraviolet Rays

PubMed Central ID

• 724514

Digital Object Identifier (DOI)

• 10.1093/nar/5.11.4263

start page

• 4263

end page

• 4272

volume

• 5

issue

• 11

URL

• http://dx.doi.org/10.1093/nar/5.11.4263