An in vitro system for accurate transcription initiation of chloroplast protein genes. Academic Article

abstract

• We have developed an homologous in vitro system from spinach chloroplasts that correctly initiates transcription of the plastid genes for the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL) and the beta subunit of the plastid ATPase (atpB). The transcriptionally active extracts from spinach chloroplasts require circular DNA templates for specific initiation. The RNA polymerase activity is insensitive to rifampicin. The extent of transcription in vitro is a function of the extract:template ratio. The efficiency of the rbcL transcription in vitro is more than one transcript per one hundred templates per hour.

authors

• Mullet, John

published proceedings

• Nucleic Acids Res

altmetric score

• 3

author list (cited authors)

• Orozco, E. M., Mullet, J. E., & Chua, N. H.

citation count

• 94

complete list of authors

• Orozco, EM||Mullet, JE||Chua, NH

publication date

• February 1985

publisher

• Oxford University Press (OUP)  Publisher

published in

• Nucleic Acids Research (NAR)  Journal

keywords

• Cell-Free System
• Chloroplasts
• DNA, Single-Stranded
• DNA-Directed RNA Polymerases
• Plant Proteins
• Plants
• Proton-translocating Atpases
• Ribulose-Bisphosphate Carboxylase
• Rifampin
• Templates, Genetic
• Transcription, Genetic

PubMed Central ID

• 2860636

Digital Object Identifier (DOI)

• 10.1093/nar/13.4.1283

start page

• 1283

end page

• 1302

volume

• 13

issue

• 4

URL

• http://dx.doi.org/10.1093/nar/13.4.1283