Application of time-resolved fluorometry to the resolution of porphyrin-photoproduct mixtures.
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Abstract—The quantitation of porphyrin and its photoproducts in biological samples is important in diverse applications including, but not limited to, tumor phototherapy. Differences in fluorescence properties, e.g., emission spectra and lifetimes, of the various components can be utilized to resolve the individual contributions. In this report time‐resolved measurements using multifrequency phase and modulation rluorometry are employed to study mixtures of protoporphyrin IX and photoprotopor‐phyrin. Individual lifetimes of 14.85 ns and 5.95 ns were determined for dioxane solutions of protoporphyrin IX and photoprotoporphyrin, respectively. These lifetime values were utilized to resolve the individual emission spectrum of each component in the mixture using the phase‐resolved Huorometry technique. Copyright © 1988, Wiley Blackwell. All rights reserved
author list (cited authors)
Valat, P., Reinhart, G. D., & Jameson, D. M
complete list of authors
Valat, P||Reinhart, GD||Jameson, DM