Dependence of ATP citrate lyase activity on cell density of isolated rat hepatocytes. Academic Article

abstract

• The activities of two enzymes involved in the lipogenic process, ATP citrate lyase and NADP-linked malic enzyme were evaluated as a function of cell density in isolated rat hepatocytes. The activity of ATP citrate lyase was profoundly affected by cell density with the activity/cell being higher at low cell densities than at high cell densities. Malic enzyme was not similarly affected, nor was cellular ATP content. The effect was observed regardless of dietary state but was most dramatic with hepatocytes from fasted-refed rats. Both an activator and an inhibitor of ATP citrate lyase have been isolated from conditioned medium from cells at low density and at high density, respectively. The activator fraction was heat stable while the inhibitor fraction was heat labile, and both factors had molecular weights in excess of 10,000 daltons.

authors

• Reinhart, Gregory

published proceedings

• Biochem Biophys Res Commun

author list (cited authors)

• Voss, A., Reinhart, G., Hogan, S., & Roehrig, K.

citation count

• 2

complete list of authors

• Voss, A||Reinhart, G||Hogan, S||Roehrig, K

publication date

• January 1987

publisher

• Elsevier  Publisher

published in

• Biochemical and Biophysical Research Communications  Journal

keywords

• Adenosine Triphosphate
• Animals
• Atp Citrate (pro-s)-lyase
• Cell Count
• Cell-Free System
• Enzyme Activation
• Fasting
• Food
• Hot Temperature
• Liver
• Malate Dehydrogenase
• Male
• Molecular Weight
• Rats
• Rats, Inbred Strains

Digital Object Identifier (DOI)

• 10.1016/0006-291x(87)91612-3

start page

• 118

end page

• 124

volume

• 149

issue

• 1

URL

• http://dx.doi.org/10.1016/0006-291x(87)91612-3