Failure of Chloro-s-triazine-Derived Compounds to Induce Estrogenic Responses in Vivo and in Vitro
Academic Article
Overview
Additional Document Info
View All
Overview
abstract
The estrogenic activity of simazine and atrazine was investigated in the immature female Sprague-Dawley rat uterus, the estrogen-responsive MCF-7 human breast cancer cell line and PL3 yeast strain. Atrazine and simazine (50 - 300 mg/kg 3) did not induce rat uterine wet weight, cytosolic progesterone receptor (PR) levels, or uterine peroxidase activity. In rats cotreated with 17-estradiol (E2) plus atrazine or simazine, there was some inhibition of E2-induced uterine PR binding and peroxidase activity. In MCF-7 cells, simazine and atrazine did not affect E2-induced cell proliferation, nuclear PR levels or luciferase activity in cells transiently transfected with the Gal4-estrogen receptor chimera and a Gal4-regulated luciferase reporter gene, and no antiestrogenic activity was observed in cotreated cells, while growth was observed on similar media supplemented with 1 nM E2. These results indicate that atrazine and simazine are not estrogenic.