PHI-X174-E COMPLEMENTS LAMBDA-S AND R-DYSFUNCTION FOR HOST-CELL LYSIS Academic Article

abstract

• Hybrid lambda phages which have the E lysis gene of the bacteriophage phi X174 in cis to defective nonsense and deletion alleles of the normal lambda lysis genes S and R have been constructed and shown to be fully competent for plaque-forming ability, which demonstrates that the single-gene, lysozyme-independent lysis system of phi X174 and related phages can serve the lytic function for large complex phages. These hybrid phages are unable to form plaques on a slyD host. Moreover, plaque morphology indicates that in E-mediated lysis the soluble lambda R endolysin can participate in lysis, indicating that the protein E-mediated lesions are not completely sealed off from the periplasm.

authors

• Young, Ryland

published proceedings

• JOURNAL OF BACTERIOLOGY

author list (cited authors)

• ROOF, W. D., & YOUNG, R.

citation count

• 11

complete list of authors

• ROOF, WD||YOUNG, R

publication date

• June 1993

publisher

• American Society for Microbiology  Publisher

keywords

• 2 Aetiology
• 2.1 Biological And Endogenous Factors
• 31 Biological Sciences
• 3102 Bioinformatics And Computational Biology
• Bacteriophage Lambda
• Bacteriophage Phi X 174
• Endopeptidases
• Genes, Viral
• Genetic Complementation Test
• Genetics
• Glycosyltransferases
• Viral Proteins
• Viral Structural Proteins
• Virus Replication

Digital Object Identifier (DOI)

• 10.1128/JB.175.12.3909-3912.1993

URI

• https://hdl.handle.net/1969.1/184445

start page

• 3909

end page

• 3912

volume

• 175

issue

• 12

URL

• http://dx.doi.org/10.1128/jb.175.12.3909-3912.1993