Lysis protein S of phage lambda functions in Saccharomyces cerevisiae. Academic Article

abstract

• The lambda S lysis gene was cloned into a Saccharomyces cerevisiae expression vector under GAL1 control. Induction with galactose in S. cerevisiae terminated cell growth and prevented colony formation. Several membrane proteins immunoreactive with anti-S antibody accumulated in the membranes, indicating that sodium dodecyl sulfate-resistant oligomers of S are formed, similar to those observed in the membranes of Escherichia coli cells killed by expression of the S gene. These observations suggest that the S gene product functions as a cytotoxic protein in the yeast cytoplasmic membrane as it does in the bacterial membrane.

authors

• Young, Ryland

published proceedings

• J Bacteriol

author list (cited authors)

• Garrett, J., Bruno, C., & Young, R.

citation count

• 22

complete list of authors

• Garrett, J||Bruno, C||Young, R

publication date

• December 1990

publisher

• American Society for Microbiology  Publisher

published in

• Journal of Bacteriology  Journal

keywords

• Amino Acid Sequence
• Bacteriolysis
• Bacteriophage Lambda
• Base Sequence
• Cloning, Molecular
• Cytotoxins
• Molecular Sequence Data
• Saccharomyces Cerevisiae
• Species Specificity
• Viral Proteins

PubMed Central ID

• 2147680

Digital Object Identifier (DOI)

• 10.1128/jb.172.12.7275-7277.1990

URI

• https://hdl.handle.net/1969.1/184444

start page

• 7275

end page

• 7277

volume

• 172

issue

• 12

URL

• http://dx.doi.org/10.1128/jb.172.12.7275-7277.1990