Lysis protein S of phage lambda functions in Saccharomyces cerevisiae. Academic Article uri icon


  • The lambda S lysis gene was cloned into a Saccharomyces cerevisiae expression vector under GAL1 control. Induction with galactose in S. cerevisiae terminated cell growth and prevented colony formation. Several membrane proteins immunoreactive with anti-S antibody accumulated in the membranes, indicating that sodium dodecyl sulfate-resistant oligomers of S are formed, similar to those observed in the membranes of Escherichia coli cells killed by expression of the S gene. These observations suggest that the S gene product functions as a cytotoxic protein in the yeast cytoplasmic membrane as it does in the bacterial membrane.

published proceedings

  • J Bacteriol

author list (cited authors)

  • Garrett, J., Bruno, C., & Young, R.

citation count

  • 22

complete list of authors

  • Garrett, J||Bruno, C||Young, R

publication date

  • December 1990