Synthesis time of beta-galactosidase in Escherichia coli B/r as a function of growth rate. Academic Article

abstract

• By analysing the kinetics of beta-galactosidase accumulation after induction, the synthesis time of beta-galactosidase in Escherichia coli B/r was found to be 75s in rapidly growing cells (1.36 and 2.1 doublings/h), and 90s in slowly growing cells (0.63 doubling/h). These values correspond to peptide-chain-elongation rates of 16 and 13 amino acids/s respectively, in agreement with previous findings, indicating that the peptide-chain growth rate is constant (presumably maximal) in fast-growing bacteria, but decreased in slowly growing bacteria [Forchhammer & Lindahl (1971) J. Mol. Biol. 55, 563-568].

authors

• Young, Ryland

published proceedings

• Biochem J

author list (cited authors)

• Dalbow, D. G., & Young, R.

citation count

• 61

complete list of authors

• Dalbow, DG||Young, R

publication date

• January 1975

publisher

• Portland Press  Publisher

published in

• Biochemical Journal  Journal

keywords

• Amino Acids
• Culture Media
• Enzyme Induction
• Escherichia Coli
• Galactosidases
• Glucose
• Kinetics
• Peptide Chain Elongation, Translational
• Succinates

Digital Object Identifier (DOI)

• 10.1042/bj1500013

start page

• 13

end page

• 20

volume

• 150

issue

• 1

URL

• http://dx.doi.org/10.1042/bj1500013