Transposition mutagenesis of bacteriophage lambda: a new gene affecting cell lysis. Academic Article

abstract

• Insertions of Tn903, a transposable kanamycin-resistance element, in bacteriophage lambda at 0.95 on the lambda physical map adversely affect growth of the phage. These insertion mutants are able to assemble particles, but are unable to lyse the infected cell properly. The mutants define a new genetic complementation group that we have designated as gene Rz. Cells infected with the Rz:: Tn903 isolates will, at the normal time of lysis, change their shape from a rod to a sphere. These spheres are stable in dilute buffers with Mg2+ but are lysed with EDTA. In addition, these results demonstrate the utility of transposition mutagenesis in refining the genetic map of even so intensely studied a genome as lambda. 1979.

authors

• Young, Ryland

published proceedings

• J Mol Biol

author list (cited authors)

• Young, R., Way, J., Way, S., Yin, J., & Syvanen, M.

citation count

• 89

complete list of authors

• Young, R||Way, J||Way, S||Yin, J||Syvanen, M

publication date

• January 1979

publisher

• Elsevier  Publisher

published in

• Journal of Molecular Biology  Journal

keywords

• Bacteriophage Lambda
• Chromosome Mapping
• DNA Transposable Elements
• DNA, Viral
• Genes, Viral
• Genetic Complementation Test
• Kanamycin
• Lysogeny
• Mutation
• R Factors
• Viral Plaque Assay

Digital Object Identifier (DOI)

• 10.1016/0022-2836(79)90262-6

start page

• 307

end page

• 322

volume

• 132

issue

• 3

URL

• http://dx.doi.org/10.1016/0022-2836(79)90262-6