Bean Yellow Dwarf Virus replicons for high‐level transgene expression in transgenic plants and cell cultures Academic Article

abstract

• A novel stable transgenic plant expression system was developed using elements of the replication machinery of Bean Yellow Dwarf Virus (BeYDV). The system contains two transgenes: 1) The BeYDV replicon vector with an expression cassette flanked by cis-acting DNA elements of BeYDV, and 2) The viral replication initiator protein (Rep) controlled by an alcohol-inducible promoter. When Rep expression was triggered by treatment with ethanol, it induced release of the BeYDV replicon from stably integrated T-DNA and episomal replication to high copy number. Replicon amplification resulted in substantially increased transgene mRNA levels (up to 80-fold) and translation products (up to 10-fold) after induction of Rep expression by ethanol treatment in tobacco NT1 cells and leaves of whole potato plants. Thus, the BeYDV stable transformant replicon system is a powerful tool for plant-based production of recombinant proteins.

authors

• Zhang, Xiuren

altmetric score

• 6

author list (cited authors)

• Zhang, X., & Mason, H.

citation count

• 58

complete list of authors

• Zhang, Xiuren||Mason, Hugh

publication date

• February 2006

publisher

• Wiley  Publisher

published in

• Biotechnology and Bioengineering  Journal

keywords

• Alcoholism, Alcohol Use And Health
• Biotechnology
• Genetics
• Substance Abuse

PubMed Central ID

• 16187337

Digital Object Identifier (DOI)

• 10.1002/bit.20695

start page

• 271

end page

• 279

volume

• 93

issue

• 2

URL

• http://dx.doi.org/10.1002/bit.20695