Optical properties of human gallbladder tissue and bile.
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abstract
Knowledge of the optical properties of biliary tract tissues is useful for aiding in the development and understanding of clinical applications of lasers in the biliary tract. Human gallbladder and bile specimens were obtained during laproscopic cholecystectomy. The diffuse remittance and transmission of the samples were determined from 350 to 2450 nm. Collimated transmission was determined at 633 and 2100 nm. Using one-dimensional diffusion theory, we calculated micro(s), micro(a), and g as a function of wavelength. Both gallbladder and bile show absorption peaks at 1.4 and 1.9 microm. Bile has a peak at 400 nm; gallbladder has peaks at 410 and 550 nm. The absorption peaks are correlated with known tissue chromophores. The effect of the sample preparation on the results is discussed.