Direct isolation of DNA from patient stools for polymerase chain reaction detection of Cryptosporidium parvum. Academic Article

abstract

• Although polymerase chain reaction (PCR) can sensitively detect parasitic or other infections, its use with fecal samples is extremely limited, primarily because of the presence of substances that inhibit DNA extension. Here an improved protocol is reported for directly isolating DNA from aged or fresh formalin-fixed stools, which can then be used to detect Cryptosporidium parvum by nested PCR. This method is highly reproducible, sensitive, and specific. It detects <1 pg of C. parvum DNA in human stool, and there are no cross-reactions with other parasites commonly found there.

authors

• Zhu, Guan

published proceedings

• J Infect Dis

altmetric score

• 3

author list (cited authors)

• Zhu, G., Marchewka, M. J., Ennis, J. G., & Keithly, J. S.

citation count

• 44

complete list of authors

• Zhu, G||Marchewka, MJ||Ennis, JG||Keithly, JS

publication date

• May 1998

publisher

• Oxford University Press (OUP)  Publisher

published in

• Journal of Infectious Diseases  Journal

keywords

• Animals
• Cryptosporidiosis
• Cryptosporidium Parvum
• DNA Primers
• DNA, Protozoan
• Diagnosis, Differential
• Enzyme-Linked Immunosorbent Assay
• Feces
• Humans
• Polymerase Chain Reaction

PubMed Central ID

• 9593044

Digital Object Identifier (DOI)

• 10.1086/517834

start page

• 1443

end page

• 1446

volume

• 177

issue

• 5

URL

• http://dx.doi.org/10.1086/517834