An RNA-seq approach to identify mechanisms by which the phytochemical sulforaphane acts to prevent prostate cancer Conference Paper uri icon


  • Prostate cancer is one of the leading causes of cancerrelated deaths in men. A phytochemical derived from cruciferous vegetables, sulforaphane (SFN), inhibits growth and induces apoptosis in prostate cancer cells, but not normal prostate epithelial cells. SFN also inhibits histone deacetylases and DNA methyltransferases, which can epigenetically affect the expression of many genes. However, the precise mechanism by which SFN acts to target cancer cells is not well understood. We utilized high throughput RNA sequencing (RNAseq) to test the hypothesis that SFN mitigates the changes in gene expression that are critical to development of prostate cancer. Normal, early androgensensitive and late androgeninsensitive prostate epithelial cells were treated with vehicle or 15uM SFN and collected at 6 and 24h post treatment. This RNAseq approach will identify global transcriptome changes in prostate epithelial cells as they progress from normal to increasingly more aggressive cancer. Furthermore, we will determine which genes are targeted by SFN in each cell line. Taken together, this will lead to identification of genes that may be critical for the prevention of prostate cancer. This information can be leveraged to develop intervention strategies to significantly reduce the incidence and severity of prostate cancer.Grant Funding Source : Funding: P01CA090890

published proceedings


author list (cited authors)

  • Beaver, L. M., Chang, J. H., Williams, D. E., Dashwood, R. H., & Ho, E.

citation count

  • 0

complete list of authors

  • Beaver, Laura Michelle||Chang, Jeff H||Williams, David E||Dashwood, Roderick H||Ho, Emily

publication date

  • April 2012