Long-term screening study on the potential toxicity of limonoids
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Twelve pregnant rats were separated into 3 equal groups. Group 1 was fed the standardized rat chow, the AIN93G diet. Groups 2 and 3 were given the same diet supplemented with a 0.25% blend of mixed limonoid glucosides (group 2) or a 0.15% blend of mixed aglycones (group 3). At weaning, 33 pups per group were selected and placed on their mother's diet. Six weeks later 15 young female rats (5/group) were bred to males in the same group. The remaining animals were sacrificed and blood samples were collected. Multiple statistical differences were found in the blood chemistries. At weaning the pups from the 15 litters were sacrificed and autopsied. The male pups from groups 2 and 3 weighed significantly less than the male pups from group 1. For the females, the pups in group 2 weighed significantly less than the pups in group 1. The data consistently showed that at this high level of exposure, that the diets with limonoids caused problems with weight gain. 2006 American Chemical Society.