FRAP analysis: accounting for bleaching during image capture. Academic Article

abstract

• The analysis of Fluorescence Recovery After Photobleaching (FRAP) experiments involves mathematical modeling of the fluorescence recovery process. An important feature of FRAP experiments that tends to be ignored in the modeling is that there can be a significant loss of fluorescence due to bleaching during image capture. In this paper, we explicitly include the effects of bleaching during image capture in the model for the recovery process, instead of correcting for the effects of bleaching using reference measurements. Using experimental examples, we demonstrate the usefulness of such an approach in FRAP analysis.

authors

• Lele, Pushkar
• Lele, Tanmay

published proceedings

• PLoS One

altmetric score

• 0.25

author list (cited authors)

• Wu, J., Shekhar, N., Lele, P. P., & Lele, T. P.

citation count

• 16

complete list of authors

• Wu, Jun||Shekhar, Nandini||Lele, Pushkar P||Lele, Tanmay P

editor list (cited editors)

• Mancini, M. A.

publication date

• August 2012

publisher

• Public Library of Science (PLoS)  Publisher

published in

• PLoS ONE  Journal

keywords

• Animals
• Fluorescence Recovery After Photobleaching
• Focal Adhesions
• Immobilized Proteins
• Mice
• Models, Theoretical
• Molecular Imaging
• NIH 3T3 Cells
• Photobleaching

PubMed Central ID

• 22912750

Digital Object Identifier (DOI)

• 10.1371/journal.pone.0042854

start page

• e42854

end page

• e42854

volume

• 7

issue

• 8

URL

• http://dx.doi.org/10.1371/journal.pone.0042854

user-defined tag

• 14 Life Below Water