Assessment of global proteome in LNCaP cells by 2D-RP/RP LC-MS/MS following sulforaphane exposure. Academic Article

abstract

• The phytochemical sulforaphane can induce cell cycle arrest and apoptosis in metastatic prostate cancer cells, though the mechanism of action is not fully known. We conducted a global proteome analysis in LNCaP metastatic prostate cancer cells to characterize how global protein signature responds to sulforaphane. We conducted parallel analyses to evaluate semi-quantitative 1-dimensional versus 2-dimensional liquid chromatography tandem mass spectrometry (LC-MS/MS) and their utility in characterizing whole cell lysate. We show that 2-dimensional LC-MS/MS can be a useful tool for characterizing global protein profiles and identify TRIAP1 as a novel regulator of cell proliferation in LNCaP metastatic prostate cancer cells.

authors

• Dashwood, Roderick

published proceedings

• EuPA Open Proteom

altmetric score

• 0.75

author list (cited authors)

• Watson, G. W., Wickramasekara, S., Maier, C. S., Williams, D. E., Dashwood, R. H., & Ho, E.

citation count

• 2

complete list of authors

• Watson, Gregory W||Wickramasekara, Samanthi||Maier, Claudia S||Williams, David E||Dashwood, Roderick H||Ho, Emily

publication date

• January 2015

publisher

• Elsevier  Publisher

published in

• EuPA Open Proteomics  Journal

keywords

• Mass Spectrometry
• Prostate Cancer
• Sulforaphane

PubMed Central ID

• 26640761

Digital Object Identifier (DOI)

• 10.1016/j.euprot.2015.08.002

start page

• 34

end page

• 40

volume

• 9

URL

• http://dx.doi.org/10.1016/j.euprot.2015.08.002