Analysis of native biological surfaces using a 100 kV massive gold cluster source. Academic Article uri icon

abstract

  • In the present work, the advantages of a new, 100 kV platform equipped with a massive gold cluster source for the analysis of native biological surfaces are shown. Inspection of the molecular ion emission as a function of projectile size demonstrates a secondary ion yield increase of ~100 for 520 keV Au(400)(4+) as compared to 130 keV Au(3)(1+) and 43 keV C(60). In particular, yields of tens of percent of molecular ions per projectile impact for the most abundant components can be observed with the 520 keV Au(400)(4+) probe. A comparison between 520 keV Au(400)(4+) time-of-flight-secondary ion mass spectrometry (TOF-SIMS) and matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) data showed a similar pattern and similar relative intensities of lipid components across a rat brain sagittal section. The abundant secondary ion yield of analyte-specific ions makes 520 keV Au(400)(4+) projectiles an attractive probe for submicrometer molecular mapping of native surfaces.

published proceedings

  • Anal Chem

author list (cited authors)

  • Fernandez-Lima, F. A., Post, J., DeBord, J. D., Eller, M. J., Verkhoturov, S. V., Della-Negra, S., Woods, A. S., & Schweikert, E. A.

citation count

  • 24

complete list of authors

  • Fernandez-Lima, Francisco A||Post, Jeremy||DeBord, John D||Eller, Michael J||Verkhoturov, Stanislav V||Della-Negra, Serge||Woods, Amina S||Schweikert, Emile A

publication date

  • November 2011