The effect of film hole row location on leading edge film cooling effectiveness and heat transfer coefficient under high mainstream turbulence conditions was experimentally determined for flow over a blunt body with semicylinder leading edge and a flat afterbody. Two separate cases of film injection film holes located only at ± 15 or ± 40 deg were studied. The holes were spaced three hole diameters apart in the spanwise direction and inclined 30 and 90 deg to the surface in the spanwise and streamwise directions, respectively. A bar grid (Tu = 5.07 percent), a passive grid (Tu = 9.67 percent), and a jet grid (Tu = 12.9 percent) produced high mainstream turbulence. The incident mainstream Reynolds number based on cylinder diameter was 100,000. Spanwise and streamwise distributions of film effectiveness and heat transfer coefficient in the leading edge and the flat sidewall were obtained for three blowing ratios. The results show mainstream turbulence adversely affects leading edge film effectiveness for the low blowing ratio (B = 0.4), but the effect reduces for higher blowing ratios (B = 0.8 and 1.2). The leading edge heat transfer coefficient increases with mainstream turbulence level for B = 0.4 and 0.8, but the effect is not systematic for B = 1.2. Mainstream turbulence effect is more severe for ±15 deg one-row injection than for ± 40 deg one-row injection. The surface heat load reduction for ± 15 deg one-row injection or ± 40 deg one-row injection is smaller than that for two-row injection.