Cell adhesion testing using novel testbeds containing micropatterns of complex nanoengineered multilayer films. Conference Paper uri icon

abstract

  • Methods for producing biomaterial patterns with defined spatial distribution micro- and nano-scale features are important for studying the cellular-level interactions, including basic cell-to-material and cell-to-cell communications. This work reports on the fabrication of substrates to study cell adhesion to multicomponent micropatterns of multilayer films by coupling conventional photolithography and LbL techniques, known as the L-LbL technique. Toward this end, substrates with nanofilm micropatterns of two different bio-functionalities have been fabricated for sPLA/sub 2/ and PLL and were used for in vitro cell-culture studies using neurons, which exhibited preferential and high efficiency and selective adhesion to sPLA/sub 2/ nanofilms. These results support the immediate use of multicomponent micropatterns as biological testbeds for basic studies of cells, and provide a basis for further expansions of the fabrication processes to produce scaffolds for precise definition of cell-to-material and cell-to-cell interactions, such that the resulting constructs mimic in vivo cell organization and behavior.

name of conference

  • The 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society

published proceedings

  • Conf Proc IEEE Eng Med Biol Soc

author list (cited authors)

  • Shaikh-Mohammed, J., DeCoster, M. A., & McShane, M. J.

citation count

  • 2

complete list of authors

  • Shaikh-Mohammed, Javeed||DeCoster, Mark A||McShane, Michael J

publication date

  • December 2004