A comparative study of sialic acid-rich proteins in rat bone and dentin. Academic Article uri icon

abstract

  • Four sialic acid-rich (SA-rich) proteins found in bone and dentin, osteopontin (OPN), bone sialoprotein (BSP), bone acidic glycoprotein-75 (BAG-75), and dentin matrix protein 1 (DMP1), share some common features. We used SDS-PAGE and Western immunoblots to analyze and compare SA-rich proteins in bone and dentin extracts from rats with a single chromatographic procedure. OPN was detected in dentin extracts, with a relative level less than one-seventieth of that in bone. Both bone and dentin BSP demonstrated an extremely broad distribution pattern, probably due to a high degree of heterogeneity in post-translational modifications. BAG-75 in both bone and dentin was detected as an 83 kDa band, dramatically distinct from that of DMPI. Using a polyclonal antibody raised against a purified bone 57 kDa protein (a portion of DMPI), we detected 150 kDa protein bands in bone fraction; the same bands were recognized by antirecombinant rat DMPI antibody. Bands from dentin migrating at about 150 kDa in earlier fractions and progressing to 200 kDa in later fractions showed a clear immunoreactivity to the anti-57 kDa antibody. We conclude that the majority of DMPI in rat bone is processed into fragments, whereas that in dentin remains intact.

published proceedings

  • Eur J Oral Sci

altmetric score

  • 3

author list (cited authors)

  • Qin, C., Brunn, J. C., Jones, J., George, A., Ramachandran, A., Gorski, J. P., & Butler, W. T.

citation count

  • 97

complete list of authors

  • Qin, C||Brunn, JC||Jones, J||George, A||Ramachandran, A||Gorski, JP||Butler, WT

publication date

  • April 2001

publisher