The expression of dentin sialophosphoprotein gene in bone. Academic Article uri icon


  • Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) are expressed as a single mRNA transcript coding for a large precursor protein termed dentin sialophosphoprotein (DSPP). DSP, DPP, and DSPP have been considered to be tooth-specific. To test for the expression of the dspp gene in bone, we performed Western immunoblots and reverse-transcription polymerase chain-reaction (RT-PCR). With Western immunoblots, we detected DSP in the Gdm/EDTA extracts of rat long bone, at a level of about 1/400 of that in dentin. Using RT-PCR, we detected DSPP mRNA in mouse calvaria. Similar to Western immunoblots, the results of RT-PCR indicated that the dspp gene is expressed at a lower level in bone than in dentin and odontoblasts. Analysis of the data shows that DSPP is not a tooth-specific protein, and that dramatically different regulatory mechanisms governing DSPP expression are involved in the bone and dentin.

published proceedings

  • J Dent Res

altmetric score

  • 3

author list (cited authors)

  • Qin, C., Brunn, J. C., Cadena, E., Ridall, A., Tsujigiwa, H., Nagatsuka, H., Nagai, N., & Butler, W. T.

citation count

  • 215

complete list of authors

  • Qin, C||Brunn, JC||Cadena, E||Ridall, A||Tsujigiwa, H||Nagatsuka, H||Nagai, N||Butler, WT

publication date

  • June 2002