Axon length quantification microfluidic culture platform for growth and regeneration study. Chapter

abstract

• To fully understand how external biomolecular environment influences axon growth, a method that can easily quantify the extent of axon growth as well as locally control their biomolecular environment is critically needed. Here, we describe a microfluidic culture platform capable of isolating CNS axons from neuronal somata for localized biomolecular manipulation as well as providing linearly guided axon growths for simple and easy quantification of the axon growth length. The axon isolation and guidance capability combined with the multi-compartment configuration make this platform ideal for investigating and screening drugs or other molecular factors that promote axon growth as well as regeneration.

authors

• Han, Arum
• Kim, Sunja
• Li, Jianrong

author list (cited authors)

• Park, J., Kim, S., Li, J., & Han, A.

citation count

• 0

complete list of authors

• Park, Jaewon||Kim, Sunja||Li, Jianrong||Han, Arum

editor list (cited editors)

• Murray, A. J.

Book Title

• AXON GROWTH AND REGENERATION: METHODS AND PROTOCOLS

publication date

• January 2014

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Axons
• Cell Culture Techniques
• Cells, Cultured
• Equipment Design
• Microfluidic Analytical Techniques
• Microscopy
• Nerve Regeneration
• Rats
• Staining And Labeling

PubMed Central ID

• 24838960

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-0777-9_7

International Standard Book Number (ISBN) 13

• 978-1-4939-0776-2

start page

• 85

end page

• 95

volume

• 1162

URL

• http://dx.doi.org/10.1007/978-1-4939-0777-9_7