Dynamical Analysis of Drug Efficacy and Mechanism of Action Using GFP Reporters Chapter uri icon


  • To the development of effective cancer drug, it is necessary to, first, identify drugs and their possible combinations that could exert desired control over the type of cancer being considered; second, have a drug testing method that allows one to assess the variety of responses that can be provoked by drugs. To facilitate such an experiment-modeling-experiment cycle for drug development, a method based on the dynamical systems of pathways is presented. It involves a three-state experimental design: (1) formulate an oncologic pathway model of relevant cancer; (2) perturb the pathways with the drugs of known effects on components of the pathways of interest; and (3) measure process activity indicators at various points on cell populations. To evaluate the drug response in a high-throughput manner, a green fluorescent protein reporter-based technology has been developed. The authors apply the dynamical approach to several issues in the context of colon cancer cell lines.

author list (cited authors)

  • Hua, J., Sima, C., Cypert, M., Dougherty, E. R., Trent, J. M., & Bittner, M. L.

complete list of authors

  • Hua, Jianping||Sima, Chao||Cypert, Milana||Dougherty, Edward R||Trent, Jeffery M||Bittner, Michael L

Book Title

  • Biometrics

publication date

  • August 2017