Studies on the Effects of Ethylene on Transformation of Tomato Cotyledons (Lycopersicon esculentum Mill.) by Agrobacterium tumefaciens
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The hypothesis that wound ethylene may be involved in transformation by Agrobacterium tumefaciens was investigated by testing the effect of ethylene inhibitors and enhancers on crown-gall tumor formation in tomato (Lycopersicon esculentum Mill.) cotyledons cultured in vitro. CoCl2 (100 μM), aminoethoxy-vinylglycine, AVG (100 μM), and aminooxyacetic acid, AOA (100 μM) reduced the quantity of ethylene released in the first 90 min after excision of tomato cotyledons. Norbornadiene (200 μmol mol-1), 1-aminocyclopropane-1-carboxylic acid, ACC (50 μM), and ethephon (50 μM) stimulated the amount of ethylene released during this time interval. There was no effect of the ethylene action inhibitor, norbornadiene, and the ethylene production inhibitor, AVG, on percent transformation. However, CoCl2 treatment completely inhibited transformation. Effects of AOA were unclear due to toxic effects of the chemical on tomato cotyledons. ACC and ethephon caused a slight reduction in percent transformation of tomato cotyledons. Ethylene seems to have little regulatory control over transformation, but the very dramatic effect of CoCl2 could indicate that an accumulation of the ethylene precursor, ACC, prevents transformation because CoCl2 inhibits conversion of ACC to ethylene. © 1992, Gustav Fischer Verlag, Stuttgart. All rights reserved.
author list (cited authors)
Davis, M. E., Miller, A. R., & Lineberger, R. D.