Rapid Micro-Assay of Camptothecin in Camptotheca acuminata Academic Article uri icon

abstract

  • A micro-assay has been developed to extract and rapidly quantify the anticancer alkaloid, camptothecin (CPT), from two leaf disks of Camptotheca acuminata Decaisne (Nyssaceae). This assay utilizes thin-layer chromatography in conjunction with fluorescence imaging to obtain reproducible measurements in the nanogram range. A large number of trees can be screened using this procedure to identify high producers of CPT in a relatively short period of time.

author list (cited authors)

  • Nolte, B. A., Lineberger, R. D., Reed, D. W., & Rumpho*, M. E.

citation count

  • 3

publication date

  • January 2001