Rapid micro-assay of camptothecin in Camptotheca acuminata. Academic Article

abstract

• A micro-assay has been developed to extract and rapidly quantify the anticancer alkaloid, camptothecin (CPT), from two leaf disks of Camptotheca acuminata Decaisne (Nyssaceae). This assay utilizes thin-layer chromatography in conjunction with fluorescence imaging to obtain reproducible measurements in the nanogram range. A large number of trees can be screened using this procedure to identify high producers of CPT in a relatively short period of time.

authors

• Lineberger, Dan
• Reed, David

published proceedings

• Planta Med

author list (cited authors)

• Nolte, B. A., Lineberger, R. D., Reed, D. W., & Rumpho, M. E.

citation count

• 3

complete list of authors

• Nolte, BA||Lineberger, RD||Reed, DW||Rumpho, ME

publication date

• January 2001

publisher

• Thieme  Publisher

published in

• Planta Medica: natural products and medicinal plant research  Journal

keywords

• Alkaloids
• Antineoplastic Agents, Phytogenic
• Camptothecin
• Chromatography, High Pressure Liquid
• Chromatography, Thin Layer
• Dna Topoisomerases, Type I
• Methylene Chloride
• Plant Extracts
• Plant Leaves
• Plants, Medicinal

PubMed Central ID

• 11458464

Digital Object Identifier (DOI)

• 10.1055/s-2001-14308

start page

• 376

end page

• 378

volume

• 67

issue

• 4

URL

• http://dx.doi.org/10.1055/s-2001-14308