Rapid micro-assay of camptothecin in Camptotheca acuminata.
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A micro-assay has been developed to extract and rapidly quantify the anticancer alkaloid, camptothecin (CPT), from two leaf disks of Camptotheca acuminata Decaisne (Nyssaceae). This assay utilizes thin-layer chromatography in conjunction with fluorescence imaging to obtain reproducible measurements in the nanogram range. A large number of trees can be screened using this procedure to identify high producers of CPT in a relatively short period of time.
author list (cited authors)
Nolte, B. A., Lineberger, R. D., Reed, D. W., & Rumpho, M. E.
complete list of authors
Nolte, BA||Lineberger, RD||Reed, DW||Rumpho, ME